Eeyore also appears in the Winnie the Pooh cartoons popularized by Disney studios. He was originally voiced by Ralph Wright, was based on Michael Liston, and his current voice actor is Peter Cullen.

Eeyore is most notable when he was ridden by Rabbit in his search for train "borrowers" in The Tigger Without A Name and The Pooh With A Name. Nearly all of Eeyore's houses in the cartoons have been bounced down. Eeyore is not good at rebuilding the houses; woozles bounce on them. Yet he soldiers on and rebuilds them time after time. Despite his depressive nature, Eeyore is capable of great compassion, which is shown when he grows a plant that Rabbit, a master gardener, was unable to grow, just by showing the plant a little love.

Eeyore has also been featured in a number of movies: Piglet's Big Movie, The Tigger Movie and Pooh's Heffalump Movie. He appears at the Walt Disney Parks and Resorts for meet and greets. His catchphrases are "Thanks for noticin' me" and "Ohhh-kayyy".

In merchandise by The Walt Disney Company, Eeyore sometimes has an uncharacteristic smile. Also, he is somewhat less caustic and sarcastic in the Disney version than in Alan Milne's original stories. His tail was not always fixed to him by a nail, although Disney has chosen this as part of his permanent image. Eeyore lost his tail in the Hundred Acre Wood. Owl found it and used it as a bell-pull beside his door, before Winnie-the-Pooh found it for Eeyore. Christopher Robin then pinned it back on. According to Winnie the Pooh and the Honey Tree, this was possible because Eeyore is full of sawdust. Also, when Eeyore appears in animation he is colored his natural grey, though he is coloured blue with a pink muzzle in merchandising.